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Rec. Antigens of Human Immunodeficiency Virus (HIV)

Synonym: HIV Rec. Antigens

Species: Human Immunodeficiency Virus

Protein Accession: AAK15879.1

Purity: Greater than 95% as determined by SDS-PAGE

Endotoxin Level: Less than 1 EU/µg

Biological Activity: The antigen is reactive with HIV-positive sera in ELISA and Western Blot.

Expression System: E. coli

Fusion Tag: None

Predicted Molecular Mass: 25 kDa

Formulation: Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4

Reconstitution: Reconstitute with sterile water to a concentration of 0.1-1.0 mg/ml.

Storage & Stability: Store at -20°C upon receipt. Stable for up to 12 months at -20°C.

FAQ

What are recombinant antigens of Human Immunodeficiency Virus (HIV)?
Recombinant antigens of HIV are proteins derived from various components of the HIV virus produced in a laboratory setting using recombinant DNA technology. These antigens mimic parts of the HIV virus and can be used for various applications, including diagnostic assays, vaccine research, and immune response studies. By using recombinant techniques, scientists can produce these antigens in large quantities and high purity, providing a reliable and consistent supply for research and diagnostic purposes. These antigens include proteins like gp120, gp41 (envelope proteins), p24 (capsid protein), and others crucial for understanding HIV and improving detection methodologies.

How are recombinant HIV antigens used in the feed industry?
While recombinant HIV antigens are primarily used in medical research and diagnostics, their role in the feed industry is generally non-existent since the feed industry traditionally focuses on animal nutrition and health. However, understanding viral antigens, in general, has implications for veterinary diagnostics and vaccine development for animal health. Similar techniques used in producing recombinant HIV antigens can be applied to create antigens for animal diseases, enhancing feed safety and the development of nutritional supplements that improve animal immune responses.

What benefits do recombinant HIV antigens offer for diagnostic tests?
Recombinant HIV antigens provide several benefits for diagnostic tests, including high specificity and sensitivity. They allow for the reliable detection of HIV infections by binding precisely to HIV antibodies in a patient's blood sample. This specificity reduces the risk of false positives and negatives, ensuring accurate test results. Additionally, recombinant antigens can be produced in large quantities and ensure batch-to-batch consistency, improving the reliability of diagnostic assays. These antigens also facilitate the development of advanced test kits like ELISAs (Enzyme-Linked Immunosorbent Assays), enhancing the early detection and monitoring of HIV in infected individuals.

Can recombinant HIV antigens be used in vaccine development?
Yes, recombinant HIV antigens are crucial in the research and development of HIV vaccines. By expressing specific HIV proteins using recombinant DNA technology, scientists create antigens that stimulate an immune response without causing infection. This approach allows researchers to study immune responses and identify which antigens and epitopes are most effective in provoking protective immunity. These insights are integral in designing vaccine candidates that train the body's immune system to recognize and combat the HIV virus more efficiently, potentially leading to effective preventive measures against the disease.

How are recombinant HIV antigens produced?
Recombinant HIV antigens are typically produced using recombinant DNA technology. The process starts with the identification and isolation of genes encoding the desired HIV proteins. These genes are then inserted into suitable expression vectors, such as plasmids, which are introduced into host cells, usually bacteria, yeast, or mammalian cells. The host cells are cultured under conditions that promote the expression of the HIV proteins. Once expressed, the proteins (antigens) are extracted and purified through a series of chromatographic and other bioseparation techniques to achieve high purity and functionality. The use of recombinant technology ensures the production of antigens with precise conformations and biological activity, essential for their effectiveness in research and diagnostic applications.

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