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Raw Materials of Ebola Virus

Synonym: Ebola Virus Antigen, EBOV Antigen

Species: Ebola Virus

Protein Accession: YP_138511.1

Purity: Greater than 95% as determined by SDS-PAGE

Endotoxin Level: Less than 1 EU/µg of protein

Biological Activity: The activity was measured by ELISA and was found to be 100ng/ml.

Expression System: E. coli

Fusion Tag: None

Predicted Molecular Mass: 30 kDa

Formulation: Sterile filtered solution in PBS

Reconstitution: Dissolve the lyophilized protein in ddH2O

Storage & Stability: Store at -80°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.

FAQ

What are the primary components of the Ebola virus relevant to the feed industry?

The Ebola virus is primarily composed of several structural proteins, genomic RNA, and a lipid envelope. Within the context of the feed industry, understanding these components can be crucial for biosecurity measures. The virus has seven structural proteins, including the nucleoprotein (NP), viral matrix proteins (VP40 and VP24), glycoprotein (GP), and polymerase complex (consisting of VP35, VP30, and the RNA-dependent RNA polymerase L). The GP is involved in binding to host cells, while NP encapsulates the RNA genome. VP40 and VP24 are associated with virion assembly and budding. These proteins, combined with the RNA strand and surrounded by a lipid envelope derived from the host cell membrane, create the infectious virus particle. Recognizing these components allows feed industry professionals to develop strategies to detect, prevent, and mitigate the risk of contamination and ensure feed and animal products' safety and biosecurity.

Can the Ebola virus affect livestock, and if so, how does it impact the feed industry?

While the Ebola virus primarily infects humans and non-human primates, there is limited evidence suggesting potential infection in specific livestock or wildlife species. However, this is not common or well-documented. In the feed industry, the paramount concern is indirect contamination through vectors such as bats, which are natural reservoirs of the virus. Contaminated feeds can pose a risk to animal health and subsequently to human health via zoonotic transmission. Measures such as stringent control of feed ingredients, proper storage conditions, regular sanitation, and monitoring for unusual animal health symptoms are vital. These protocols can reduce the risk of cross-contamination and ensure the production of safe feed products.

What biosecurity measures should the feed industry implement to prevent Ebola virus contamination?

To prevent Ebola virus contamination, the feed industry needs to adopt robust biosecurity measures. Firstly, sourcing raw materials from reputable and certified suppliers is essential. Regular inspections and audits of suppliers can ensure compliance with safety standards. Secondly, implementing strict hygiene and sanitation protocols within manufacturing facilities, including frequent disinfection of equipment and storage areas, helps minimize contamination risks. Personnel should be trained in biosecurity protocols and equipped with appropriate personal protective equipment (PPE). Testing raw materials and finished feed products for pathogens can provide an additional safety layer. Finally, establishing a surveillance system for early detection of potential Ebola virus presence and conducting regular risk assessments can help in taking proactive measures to safeguard feed production.

What role does feed ingredient sourcing play in preventing Ebola virus contamination?

Sourcing feed ingredients from reputable suppliers plays a crucial role in preventing Ebola virus contamination. Suppliers who adhere to stringent health and safety standards reduce the risk of introducing contaminated ingredients into the feed supply chain. This includes ensuring that sourced ingredients are free from contact with natural reservoirs of the virus, such as bats, or other vectors. Transparent supply chain management and traceability enable quick identification and isolation of potentially contaminated batches. Regular audits and assessments of suppliers for compliance with biosecurity measures are essential. Establishing long-term relationships with trusted suppliers can build a reliable supply chain that prioritizes quality and safety, ultimately reducing the risk of Ebola virus contamination in feed products.

How can the detection of Ebola virus in feed materials be carried out effectively?

Effective detection of the Ebola virus in feed materials involves several methodological approaches. Molecular techniques, such as reverse transcription polymerase chain reaction (RT-PCR), are highly sensitive and specific for identifying viral RNA. This method can be used to screen samples from raw materials and final feed products. Serological tests, which detect antibodies or antigens related to the virus, can be utilized for broader surveillance. Environmental monitoring, including swabs from storage and processing areas, can help identify contamination sources. Innovative approaches like next-generation sequencing (NGS) offer comprehensive insights into pathogen presence. Establishing routine testing protocols and collaborating with specialized laboratories ensure early detection and containment, safeguarding the feed supply chain from potential Ebola virus contamination.

Jiangsu East-Mab Biomedical Technology Co.,Ltd

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