Synonym: UDG
Species: Escherichia coli
Protein Accession: P06744
Purity: Greater than 95% by SDS-PAGE
Endotoxin Level: Less than 1 EU/μg
Biological Activity: Removes uracil from single-stranded or double-stranded DNA
Expression System: E. coli
Fusion Tag: 6xHis tag
Predicted Molecular Mass: 24.9 kDa
Formulation: Supplied in 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT
and 50% glycerol
Reconstitution: It is recommended to reconstitute the lyophilized UDG in sterile
water
Storage & Stability: Store at -20°C. For long-term storage, store at -80°C. Stable
for 12 months from the date of receipt when stored at -20°C to -80°C
FAQ
What is EB0302 Uracil-DNA Glycosylase, and how does it benefit the feed
industry?
EB0302 Uracil-DNA Glycosylase (UDG) is an enzyme that specifically targets
and excises uracil residues incorporated into DNA. In the context of the feed industry,
this enzyme can be utilized to enhance the accuracy of DNA-based diagnostic tools for
pathogen detection and quality control. By removing uracil, UDG helps maintain the
integrity of these diagnostic assays, ensuring precise identification of harmful
pathogens in feed products, which, in turn, supports the health and safety of livestock.
Additionally, UDG can play a vital role in the production of genetically modified feed
crops by ensuring genomic stability and preventing mutations that could affect crop
yield and quality.
How can EB0302 Uracil-DNA Glycosylase improve feed quality
control procedures?
In the feed industry, quality control is paramount to ensure the
safety and efficacy of feed products. EB0302 UDG assists in enhancing these procedures
by improving the reliability of DNA-based assays used for pathogen detection and genetic
verification of feed ingredients. By removing uracil residues from DNA, UDG prevents
false-positive results, which can occur due to the presence of uracil. This ensures that
any detected pathogens are accurately identified, and feed products meet stringent
safety standards. Consequently, it reduces the risk of contamination, improves livestock
health, and enhances overall feed quality.
What applications does EB0302
Uracil-DNA Glycosylase have in pathogen detection for feed safety?
EB0302 UDG is
integral to pathogen detection systems that rely on DNA analysis. In feed safety,
accurate detection of pathogens such as Salmonella, E. coli, and other harmful
microorganisms is crucial. UDG enhances the precision of polymerase chain reaction (PCR)
and other DNA-based assays by excising uracil from DNA templates, thus preventing
erroneous detection results. This ensures that only true pathogen DNA is amplified and
detected, providing more reliable and timely identification of contaminants in feed
products. Rapid and accurate pathogen detection helps mitigate risks and ensures the
maintenance of high safety standards in the feed industry.
How does EB0302
Uracil-DNA Glycosylase support the production of genetically modified feed
crops?
EB0302 UDG supports the production of genetically modified (GM) feed crops by
ensuring the stability and integrity of the genetic material. In GM crop development,
genetic modifications are introduced to achieve desired traits such as pest resistance
or increased nutritional value. UDG removes uracil residues from the DNA, which can be
incorporated during genetic engineering and could lead to mutations if not excised. By
ensuring accurate and stable genetic sequences, UDG contributes to consistent crop
performance and trait expression. This reliability is essential for the successful
commercialization of GM feed crops, enabling the feed industry to provide enhanced
nutritional solutions to livestock producers.
Can EB0302 Uracil-DNA Glycosylase
be used in animal nutrition research?
Yes, EB0302 UDG can be utilized extensively in
animal nutrition research. It plays a critical role in molecular biology applications
used to study the genetic and microbial components of animal nutrition. For instance,
researchers often analyze microbial communities in the gut to understand their impact on
digestion and nutrient absorption. UDG helps by ensuring the accuracy of genetic
sequencing and PCR-based studies, which are used to profile these microbial communities.
Accurate genetic data obtained with the help of UDG can lead to better insights into
optimal feed formulations and the development of probiotic and prebiotic supplements,
ultimately enhancing animal health and productivity in the feed industry.