Product Detail

Current Location: Home> Industry Enzymes>Other Enzymes>EB0302 Uracil-DNA Glycosylase
EB0302 Uracil-DNA Glycosylase

Synonym: UDG

Species: Escherichia coli

Protein Accession: P06744

Purity: Greater than 95% by SDS-PAGE

Endotoxin Level: Less than 1 EU/μg

Biological Activity: Removes uracil from single-stranded or double-stranded DNA

Expression System: E. coli

Fusion Tag: 6xHis tag

Predicted Molecular Mass: 24.9 kDa

Formulation: Supplied in 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT and 50% glycerol

Reconstitution: It is recommended to reconstitute the lyophilized UDG in sterile water

Storage & Stability: Store at -20°C. For long-term storage, store at -80°C. Stable for 12 months from the date of receipt when stored at -20°C to -80°C

FAQ

What is EB0302 Uracil-DNA Glycosylase, and how does it benefit the feed industry?
EB0302 Uracil-DNA Glycosylase (UDG) is an enzyme that specifically targets and excises uracil residues incorporated into DNA. In the context of the feed industry, this enzyme can be utilized to enhance the accuracy of DNA-based diagnostic tools for pathogen detection and quality control. By removing uracil, UDG helps maintain the integrity of these diagnostic assays, ensuring precise identification of harmful pathogens in feed products, which, in turn, supports the health and safety of livestock. Additionally, UDG can play a vital role in the production of genetically modified feed crops by ensuring genomic stability and preventing mutations that could affect crop yield and quality.

How can EB0302 Uracil-DNA Glycosylase improve feed quality control procedures?
In the feed industry, quality control is paramount to ensure the safety and efficacy of feed products. EB0302 UDG assists in enhancing these procedures by improving the reliability of DNA-based assays used for pathogen detection and genetic verification of feed ingredients. By removing uracil residues from DNA, UDG prevents false-positive results, which can occur due to the presence of uracil. This ensures that any detected pathogens are accurately identified, and feed products meet stringent safety standards. Consequently, it reduces the risk of contamination, improves livestock health, and enhances overall feed quality.

What applications does EB0302 Uracil-DNA Glycosylase have in pathogen detection for feed safety?
EB0302 UDG is integral to pathogen detection systems that rely on DNA analysis. In feed safety, accurate detection of pathogens such as Salmonella, E. coli, and other harmful microorganisms is crucial. UDG enhances the precision of polymerase chain reaction (PCR) and other DNA-based assays by excising uracil from DNA templates, thus preventing erroneous detection results. This ensures that only true pathogen DNA is amplified and detected, providing more reliable and timely identification of contaminants in feed products. Rapid and accurate pathogen detection helps mitigate risks and ensures the maintenance of high safety standards in the feed industry.

How does EB0302 Uracil-DNA Glycosylase support the production of genetically modified feed crops?
EB0302 UDG supports the production of genetically modified (GM) feed crops by ensuring the stability and integrity of the genetic material. In GM crop development, genetic modifications are introduced to achieve desired traits such as pest resistance or increased nutritional value. UDG removes uracil residues from the DNA, which can be incorporated during genetic engineering and could lead to mutations if not excised. By ensuring accurate and stable genetic sequences, UDG contributes to consistent crop performance and trait expression. This reliability is essential for the successful commercialization of GM feed crops, enabling the feed industry to provide enhanced nutritional solutions to livestock producers.

Can EB0302 Uracil-DNA Glycosylase be used in animal nutrition research?
Yes, EB0302 UDG can be utilized extensively in animal nutrition research. It plays a critical role in molecular biology applications used to study the genetic and microbial components of animal nutrition. For instance, researchers often analyze microbial communities in the gut to understand their impact on digestion and nutrient absorption. UDG helps by ensuring the accuracy of genetic sequencing and PCR-based studies, which are used to profile these microbial communities. Accurate genetic data obtained with the help of UDG can lead to better insights into optimal feed formulations and the development of probiotic and prebiotic supplements, ultimately enhancing animal health and productivity in the feed industry.

Jiangsu East-Mab Biomedical Technology Co.,Ltd

contact us

Factory address:
Life and Health Industrial Park, Rudong County, Nantong City, Jiangsu Province

Contact: Fred Wang
Tel: 0086-25-52397805
Phone/WhatsApp: 0086-18005193850

mobile
Jiangsu East-Mab Biomedical Technology Co.,Ltd

Copyright(C)2023,Jiangsu East-Mab Biomedical Technology Co.,Ltd All Rights Reserved.

Leave A Message
Leave A Message ×
If you are interested in our products and want to know more details,please leave a message here,we will reply you as soon as we can.