Synonym: Bst DNA Polymerase
Species: Bacillus stearothermophilus
Protein Accession: EB0201
Purity: ≥95%
Endotoxin Level:
<0.1 EU/μg
Biological Activity: Amplification of DNA fragments up to 30 kb
Expression System: E. coli
Fusion Tag: None
Predicted Molecular Mass: 94 kDa
Formulation: Supplied in 20 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM
DTT, 50% Glycerol, pH 8.0
Reconstitution: Add deionized water to prepare working solutions
Storage & Stability: Store at -20°C. Stable for 1 year at -20°C
FAQ
What is EB0201 Bst DNA Polymerase, and how does it work in the feed
industry?
EB0201 Bst DNA Polymerase is a high-performance enzyme used
extensively in the feed industry for DNA amplification processes such as
loop-mediated isothermal amplification (LAMP). Its primary role is to facilitate
rapid and efficient amplification of DNA at a constant temperature,
significantly enhancing the detection and quantification of various genetic
elements present in feed and feed ingredients. This capability is crucial for
quality control, ensuring feed safety, and monitoring for the presence of
genetically modified organisms (GMOs) or pathogenic contaminants. By providing
robust and reliable performance, EB0201 Bst DNA Polymerase helps in maintaining
stringent feed quality standards.
What are the key features of EB0201 Bst
DNA Polymerase that make it suitable for feed industry
applications?
EB0201 Bst DNA Polymerase boasts several features ideal for
feed industry applications. Firstly, it is highly processive and thermally
stable, enabling it to work efficiently at consistent high temperatures. This
stability reduces reaction times and increases throughput in testing
laboratories. Additionally, the enzyme exhibits strong strand displacement
activity, which is essential for LAMP and other isothermal amplification
techniques, allowing for more effective DNA amplification. Its high fidelity
ensures accurate replication of target sequences, crucial for precise
identification and analysis. Moreover, EB0201 Bst DNA Polymerase functions in
diverse and complex sample matrices typical in the feed industry, ensuring
reliability across varied sample types.
How does the use of EB0201 Bst
DNA Polymerase improve the safety and quality control of feed
products?
The use of EB0201 Bst DNA Polymerase in the feed industry
significantly enhances the safety and quality control of feed products through
its efficient DNA amplification capabilities. This enzyme enables rapid and
accurate detection of potential contaminants such as mycotoxins, pathogenic
bacteria, and viruses, as well as GMOs. By providing fast and reliable results,
it allows for timely interventions to prevent contaminated feed from reaching
livestock, thus safeguarding animal health. Additionally, it supports the
traceability of feed ingredients, ensuring adherence to regulatory standards and
facilitating audits and certifications. Overall, it helps feed producers
maintain high quality and safety standards consistently.
Can EB0201 Bst
DNA Polymerase be used for GMO detection in feed products, and what are the
benefits of such applications?
Yes, EB0201 Bst DNA Polymerase is highly
effective for GMO detection in feed products. Its ability to amplify DNA at a
constant temperature makes it particularly suited for LAMP assays, which are
efficient and user-friendly methods for detecting GMOs. The benefits of using
EB0201 Bst DNA Polymerase for GMO detection include enhanced sensitivity and
specificity, enabling the identification of even low levels of GMO content. This
is vital for regulatory compliance and for meeting export requirements in
markets with strict GMO regulations. Furthermore, the rapid testing facilitated
by this enzyme allows for quick decision-making in the production process,
preventing the inclusion of unauthorized GMOs in feed products.
What
considerations should be taken into account when implementing EB0201 Bst DNA
Polymerase in feed industry laboratories?
When implementing EB0201 Bst
DNA Polymerase in feed industry laboratories, several considerations are
essential. First, ensure that laboratory personnel are adequately trained in
using isothermal amplification techniques such as LAMP, as these methods differ
from traditional PCR. Laboratories should also validate their testing protocols
to confirm the enzyme's performance with various feed samples and matrixes.
Proper calibration of equipment and adherence to standardized procedures are
crucial for maintaining accuracy and reliability. Additionally, the lab should
have robust quality control measures in place, including the use of positive and
negative controls to monitor for potential contamination or reaction errors.
Lastly, consider the storage and handling requirements of EB0201 Bst DNA
Polymerase to maintain its activity and efficacy over time.