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EB0201 Bst DNA Polymerase

Synonym: Bst DNA Polymerase

Species: Bacillus stearothermophilus

Protein Accession: EB0201

Purity: ≥95%

Endotoxin Level: <0.1 EU/μg

Biological Activity: Amplification of DNA fragments up to 30 kb

Expression System: E. coli

Fusion Tag: None

Predicted Molecular Mass: 94 kDa

Formulation: Supplied in 20 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol, pH 8.0

Reconstitution: Add deionized water to prepare working solutions

Storage & Stability: Store at -20°C. Stable for 1 year at -20°C

FAQ

What is EB0201 Bst DNA Polymerase, and how does it work in the feed industry?

EB0201 Bst DNA Polymerase is a high-performance enzyme used extensively in the feed industry for DNA amplification processes such as loop-mediated isothermal amplification (LAMP). Its primary role is to facilitate rapid and efficient amplification of DNA at a constant temperature, significantly enhancing the detection and quantification of various genetic elements present in feed and feed ingredients. This capability is crucial for quality control, ensuring feed safety, and monitoring for the presence of genetically modified organisms (GMOs) or pathogenic contaminants. By providing robust and reliable performance, EB0201 Bst DNA Polymerase helps in maintaining stringent feed quality standards.

What are the key features of EB0201 Bst DNA Polymerase that make it suitable for feed industry applications?

EB0201 Bst DNA Polymerase boasts several features ideal for feed industry applications. Firstly, it is highly processive and thermally stable, enabling it to work efficiently at consistent high temperatures. This stability reduces reaction times and increases throughput in testing laboratories. Additionally, the enzyme exhibits strong strand displacement activity, which is essential for LAMP and other isothermal amplification techniques, allowing for more effective DNA amplification. Its high fidelity ensures accurate replication of target sequences, crucial for precise identification and analysis. Moreover, EB0201 Bst DNA Polymerase functions in diverse and complex sample matrices typical in the feed industry, ensuring reliability across varied sample types.

How does the use of EB0201 Bst DNA Polymerase improve the safety and quality control of feed products?

The use of EB0201 Bst DNA Polymerase in the feed industry significantly enhances the safety and quality control of feed products through its efficient DNA amplification capabilities. This enzyme enables rapid and accurate detection of potential contaminants such as mycotoxins, pathogenic bacteria, and viruses, as well as GMOs. By providing fast and reliable results, it allows for timely interventions to prevent contaminated feed from reaching livestock, thus safeguarding animal health. Additionally, it supports the traceability of feed ingredients, ensuring adherence to regulatory standards and facilitating audits and certifications. Overall, it helps feed producers maintain high quality and safety standards consistently.

Can EB0201 Bst DNA Polymerase be used for GMO detection in feed products, and what are the benefits of such applications?

Yes, EB0201 Bst DNA Polymerase is highly effective for GMO detection in feed products. Its ability to amplify DNA at a constant temperature makes it particularly suited for LAMP assays, which are efficient and user-friendly methods for detecting GMOs. The benefits of using EB0201 Bst DNA Polymerase for GMO detection include enhanced sensitivity and specificity, enabling the identification of even low levels of GMO content. This is vital for regulatory compliance and for meeting export requirements in markets with strict GMO regulations. Furthermore, the rapid testing facilitated by this enzyme allows for quick decision-making in the production process, preventing the inclusion of unauthorized GMOs in feed products.

What considerations should be taken into account when implementing EB0201 Bst DNA Polymerase in feed industry laboratories?

When implementing EB0201 Bst DNA Polymerase in feed industry laboratories, several considerations are essential. First, ensure that laboratory personnel are adequately trained in using isothermal amplification techniques such as LAMP, as these methods differ from traditional PCR. Laboratories should also validate their testing protocols to confirm the enzyme's performance with various feed samples and matrixes. Proper calibration of equipment and adherence to standardized procedures are crucial for maintaining accuracy and reliability. Additionally, the lab should have robust quality control measures in place, including the use of positive and negative controls to monitor for potential contamination or reaction errors. Lastly, consider the storage and handling requirements of EB0201 Bst DNA Polymerase to maintain its activity and efficacy over time.

Jiangsu East-Mab Biomedical Technology Co.,Ltd

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