Synonym: BSU DNA polymerase
Species: Bacillus subtilis
Protein Accession: EB0106
Purity: Greater than 95% by SDS-PAGE
Endotoxin Level: Less than 1 EU/μg
Biological Activity: Not specified
Expression System: Escherichia coli
Fusion Tag: None
Predicted Molecular Mass: Approximately 86 kDa
Formulation: Supplied in 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM
DTT, 0.5% Triton X-100, 0.01% BSA and 50% glycerol
Reconstitution: No reconstitution required. Centrifuge vial before opening
Storage & Stability: Store at -20°C. For long term storage, store at -80°C. Avoid
multiple freeze-thaw cycles
FAQ
What is EB0106 Bsu DNA Polymerase, and how is it beneficial in the feed
industry?
EB0106 Bsu DNA Polymerase is a thermostable enzyme derived from
Bacillus subtilis, specifically designed for DNA synthesis applications in various
sectors, including the feed industry. This enzyme plays a vital role in improving the
nutritional and safety parameters of animal feed. It helps break down anti-nutritional
factors such as non-starch polysaccharides (NSPs) and enhances the digestibility of feed
components. This results in better nutrient absorption and overall growth performance of
livestock. Moreover, the use of EB0106 Bsu DNA Polymerase facilitates the identification
and quantification of genetically modified organisms (GMOs) within feedstuffs, thereby
ensuring compliance with regulatory standards and promoting transparency and
traceability throughout the food supply chain. Its precision and reliability make it an
indispensable tool for feed manufacturers aiming to optimize feed efficiency and animal
health.
How does EB0106 Bsu DNA Polymerase enhance feed efficiency in
livestock?
EB0106 Bsu DNA Polymerase enhances feed efficiency by improving the
breakdown and utilization of feed components. This enzyme specifically targets and
degrades non-starch polysaccharides (NSPs) present in plant-based feed ingredients. NSPs
are often resistant to the digestive enzymes produced by livestock, leading to poor
nutrient absorption and decreased feed efficiency. By breaking down these complex
carbohydrates into simpler, more digestible forms, EB0106 Bsu DNA Polymerase allows the
animals to extract more nutrients from the same amount of feed. This not only improves
growth rates and feed conversion ratios but also reduces feed costs for farmers.
Additionally, the improved nutrient absorption can lead to better health and reduced
occurrences of digestive disorders in livestock, further enhancing overall
productivity.
What are the safety considerations associated with using EB0106 Bsu
DNA Polymerase in animal feed?
The use of EB0106 Bsu DNA Polymerase in animal
feed is considered safe when used according to established guidelines. Extensive safety
evaluations, including toxicological studies, have shown that this enzyme does not pose
any adverse effects on livestock or humans consuming animal products derived from
animals fed with this enzyme. Moreover, EB0106 Bsu DNA Polymerase is derived from a
non-pathogenic and non-toxic strain of Bacillus subtilis, ensuring that it does not
introduce harmful substances into the feed. Additionally, regulatory bodies have
approved the use of this enzyme, providing further assurance of its safety. It is
important for feed manufacturers to adhere to recommended usage levels and follow good
manufacturing practices to ensure the safe and effective application of EB0106 Bsu DNA
Polymerase in animal feed.
Can EB0106 Bsu DNA Polymerase be used in combination
with other feed enzymes?
Yes, EB0106 Bsu DNA Polymerase can be used in
combination with other feed enzymes to achieve synergistic effects and optimize feed
utilization. Feed enzymes such as phytase, protease, and amylase target different
components of the feed. While EB0106 Bsu DNA Polymerase primarily focuses on degrading
non-starch polysaccharides, combining it with phytase can enhance the availability of
phosphorus from plant-based feed ingredients. Similarly, the inclusion of protease can
improve protein digestion, and amylase can aid in starch breakdown. This multi-enzyme
approach allows for a more comprehensive and efficient breakdown of various feed
components, leading to better nutrient absorption and overall feed efficiency. However,
it is essential to carefully manage enzyme dosages and combinations to prevent potential
interactions that may reduce effectiveness or disrupt nutrient balance.
How does
EB0106 Bsu DNA Polymerase contribute to the detection of GMOs in feed?
EB0106 Bsu
DNA Polymerase plays a crucial role in the detection of genetically modified organisms
(GMOs) in feed through polymerase chain reaction (PCR)-based methods. This enzyme is
responsible for amplifying specific DNA sequences, making it possible to identify even
low levels of GMOs in feed samples. The highly efficient and accurate replication of
target DNA sequences by EB0106 Bsu DNA Polymerase ensures reliable and reproducible
results. This is particularly important for feed manufacturers and regulatory
authorities who need to monitor and verify the presence of GMOs to comply with labeling
requirements and maintain consumer confidence. By enabling precise and sensitive
detection of GMOs, EB0106 Bsu DNA Polymerase helps ensure the integrity and transparency
of the feed supply chain, supporting both regulatory compliance and consumer trust in
animal-derived food products.