Synonym: LIF
Species: Human
Protein Accession: P15018
Purity: Greater than 98% as determined by SDS-PAGE
Endotoxin Level: Less than 0.1 ng/ug (1EU/ug)
Biological Activity: The ED50 for this effect is typically 0.2-1.0 ng/mL.
Expression System: E. coli
Fusion Tag: None
Predicted Molecular Mass: Approximately 20.8 kDa
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4
Reconstitution: It is recommended to reconstitute the lyophilized Human LIF in
sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other
aqueous solutions.
Storage & Stability: Store lyophilized product at -20°C. Reconstitution can be
stored at 4°C for up to 2 weeks. For long term storage, aliquot and store at -20°C.
Avoid repeated freeze-thaw cycles.
FAQ
What is Y03001 Recombinant Human LIF and how is it beneficial for the feed
industry?
Y03001 Recombinant Human Leukemia Inhibitory Factor (LIF) is a cytokine
belonging to the interleukin-6 family, known for its multifaceted biological activities,
including cell differentiation, proliferation, and survival. In the context of the feed
industry, LIF can be particularly beneficial due to its ability to enhance growth rates,
improve immune response, and increase overall health in livestock. This cytokine helps
in optimizing feed efficiency, thereby reducing the cost of production and improving the
economic value of livestock farming. By promoting a healthier and more robust immune
system, animals can better resist infections and diseases, leading to reduced use of
antibiotics and other medications, which is crucial for maintaining food safety
standards. Additionally, LIF's role in cellular health and development can result in
better meat quality and yield, providing a competitive edge in the marketplace.
How does Y03001 Recombinant Human LIF improve the immune response in
livestock?
LIF plays a critical role in modulating the immune system by
influencing the activity of various immune cells. It enhances the production and
function of leukocytes, particularly macrophages and lymphocytes, which are essential
for a robust immune response. By stimulating these cells, LIF improves the animal's
ability to fend off pathogens and recover from infections more swiftly. This is
particularly valuable in intensive farming environments where animals are in close
quarters and more susceptible to diseases. LIF also has anti-inflammatory properties
that help in reducing the overall inflammatory load on the animal, promoting quicker
recovery and maintaining overall health. The use of LIF in feed can, therefore, reduce
veterinary costs and minimize the loss associated with disease outbreaks, contributing
to more sustainable livestock management.
In what ways can Y03001 Recombinant
Human LIF enhance growth rates in livestock?
LIF impacts growth by influencing
various cellular pathways that regulate cell proliferation, differentiation, and
metabolism. When included in feed, LIF can enhance the growth hormone signaling pathways
and increase the expression of genes involved in muscle development and fat metabolism.
This results in not only faster growth rates but also an improved feed conversion ratio
(FCR), meaning animals require less feed to gain the same amount of weight. Improved
growth rates also translate to shorter time to market, optimizing production cycles and
increasing potential revenue. The integration of LIF in animal feed ensures that the
livestock grow healthier and stronger, presenting a dual benefit of increased
productivity and enhanced animal welfare.
What are the potential safety concerns
associated with Y03001 Recombinant Human LIF in animal feed?
The safety of Y03001
Recombinant Human LIF in animal feed is a primary consideration, and comprehensive
studies must be conducted to ensure its non-toxicity and efficacy. Generally,
recombinant proteins like LIF are produced using biotechnological methods that ensure
high purity and minimal contaminants. It's crucial to adhere to regulatory guidelines
and conduct rigorous trials to assess any adverse effects. Given its high homology with
naturally occurring LIF, the risk of negative reactions is relatively low. However,
continuous monitoring and assessment will ensure safe and effective integration into
feed protocols. Proper dosing and administration guidelines should be followed to avoid
potential issues like overstimulation of the immune system, which could lead to
autoimmune responses or other imbalances.
Can the application of Y03001
Recombinant Human LIF replace antibiotics in livestock production?
Y03001
Recombinant Human LIF holds potential as a natural alternative to antibiotics by
bolstering the immune system and improving overall health, thereby reducing the
necessity for antibiotic interventions. Its role in enhancing the animal's natural
defenses means that livestock can more effectively fight off infections, mitigating the
spread of diseases. This is particularly important given the global push to reduce
antibiotic use to combat antimicrobial resistance (AMR). While LIF may not entirely
replace the need for antibiotics, its inclusion in feed can significantly reduce
dependency on them, promoting a more sustainable and responsible approach to livestock
management. It is crucial to adopt a holistic view, considering LIF as part of an
integrated disease management strategy, including good farm hygiene, regular health
monitoring, and proper nutrition.